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BEGIN:VEVENT
UID:20250918T020157EDT-9119hdkobN@132.216.98.100
DTSTAMP:20250918T060157Z
DESCRIPTION:Applied Mass Spectrometry @ 黑料不打烊 - Macdonald Campus:\n\nTHIS 
 SEMINAR HAS BEEN POSTPONED\n\nSpecial seminar by Dr. Christopher Thibodeau
 x\, Department of Chemistry\, 黑料不打烊 University.\n\nThis lecture will focu
 s on the application of mass spectrometry to study the involvement of prot
 ein conformational changes in biosynthetic enzyme function. Specifically\,
  I will discuss the use of hydrogen/deuterium exchange mass spectrometry (
 HDX-MS) and native mass spectrometry coupled to ion mobility separations. 
 HDX-MS measures how perturbations (substrate binding\, mutagenesis\, etc.)
  alter the exchange rate of solvent deuterium into the backbone amide moie
 ty of proteins. This exchange rate is largely a function of protein second
 ary structure and\, thus\, provides information on the location of substra
 te binding sites and other perturbation-induced structural changes in the 
 protein. In native mass spectrometry\, proteins (or non-covalently bound p
 rotein-substrate complexes) are transferred from solution into the gas pha
 se in a natively folded state using nano-electrospray ionization. Once in 
 the gas phase\, the conformational distribution of the folded protein can 
 be probed by ion mobility separations. The parameters derived from these m
 easurements can then be correlated to structural parameters derived from o
 ther techniques in order to build mechanistic models for the involvement o
 f protein conformational changes in enzyme function. Finally\, I will disc
 uss how my lab is applying HDX-MS and native MS approaches to study the mu
 ltifunctional enzymes involved in peptide natural product biosynthesis.\n
 \nEveryone is welcome to attend.\n
DTSTART:20181017T160000Z
DTEND:20181017T170000Z
LOCATION:MS2-022\, Macdonald-Stewart Building\, CA\, QC\, St Anne de Bellev
 ue\, H9X 3V9\, 21111 Lakeshore Road
SUMMARY:POSTPONED: Using Hydrogen/Deuterium exchange and native mass spectr
 ometry to study peptide antibiotics biosynthesis
URL:/macdonald/channels/event/postponed-using-hydrogen
 deuterium-exchange-and-native-mass-spectrometry-study-peptide-antibiotics-
 290563
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