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UID:20250731T042131EDT-34587WjXES@132.216.98.100
DTSTAMP:20250731T082131Z
DESCRIPTION:Applied Mass Spectrometry [food/nutrition/environment and agric
 ulture] @ ºÚÁϲ»´òìÈ - Macdonald Campus\n\nGuest speaker: Prof Pierre Chaurand
 \, Department of Chemistry\, Université de Montréal\n\nImaging mass spectr
 ometry (IMS) has become the leading technique for the analysis of biomolec
 ules directly from thin tissue sections while retaining the biomolecules’ 
 anatomical distributions. In recent years\, MALDI IMS as greatly improved 
 in spatial resolution due to the advancement in both sample preparations a
 nd instruments to the point of reaching cellular level capabilities. In th
 is workshop\, after giving an overview of IMS technology and of its potent
 ial\, new developments in IMS from our laboratory to increase specificity 
 and sensitivity will be presented. More specifically\, matrix-free metal a
 ssisted LDI IMS methods have been developed for the specific analysis of n
 eutral lipid compounds. For these\, nanometer thin metal layers are direct
 ly sputter deposited on tissue sections. From the investigation of various
  tissue specimens\, using silver-assisted LDI IMS we have thus far mapped 
 and identified several compounds including\, cholesterol\, arachidonic aci
 d and docosahexaenoic acid. While both fatty acids and cholesterol show gr
 eat affinity for silver\, other molecules such as triglycerides have stron
 g affinities for sodium cations. For this purpose\, the coupling of sodium
  salts and sputtered layers of gold has been developed to enhance both des
 orption and ionization of triglycerides yielding a 30-fold increase in sig
 nal compared to standard MALDI MS approaches. Although gold-assisted LDI I
 MS targets triglycerides\, we have also detected and imaged several other 
 compounds including cholesterol esters\, which are hard to detect by MALDI
  MS. The clinical potentials of these novel IMS strategies for the detecti
 on and characterization of molecular changes in disease has been exemplifi
 ed through studies of Alzheimer’s and non-alcoholic fatty liver diseases.
 \n\nABOUT THE SPEAKER\n\nPierre Chaurand received his Ph.D. in physical ch
 emistry from the Université Paris Sud\, (Orsay\, France) in 1994. After a 
 postdoctoral period at the Heinrich-Heine Universität\, (Düsseldorf\, Germ
 any)\, he integrated in 1998 the laboratory of Prof. Richard Caprioli as R
 esearch Faculty at Vanderbilt University School of Medicine (Nashville TN\
 , USA). Since 2009\, he is Professor in the Department of Chemistry at the
  Université de Montréal (Montreal\, Canada). His primary background is in 
 biochemistry with significant analytical and organic chemistry components.
  His current expertise’s are in fundamental and analytical mass spectromet
 ry. With over 20 years of experience in the field\, he is one of the pione
 ers of the imaging mass spectrometry (IMS) technology. His current researc
 h interests are focused on the development of new strategies and methods t
 o improve the specificity and sensitivity of tissue based IMS with applica
 tions in fundamental and clinical biology.\n\nEveryone is welcome to atten
 d.\n
DTSTART:20190221T163000Z
DTEND:20190221T173000Z
LOCATION:R4-045\, Raymond Building\, CA\, QC\, St Anne de Bellevue\, H9X 3V
 9\, 21111 Lakeshore Road
SUMMARY:Novel sample preparation approaches for LDI imaging MS to answer ke
 y biological questions
URL:/macdonald/channels/event/novel-sample-preparation
 -approaches-ldi-imaging-ms-answer-key-biological-questions-294557
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